Weaning pigs often face post-weaning challenges such as diarrhea, low feed intake, and body weight (BW) loss which affects the health and economic value of weaning pigs. Interestingly, the use of yeast cultures (YCs) as feed supplements for pigs has increased markedly in recent years. This study evaluated the effects of yeast cultures (Saccharomyces cerevisiae) on the growth performance, fecal score, and nutrient digestibility of weaning pigs. A total of 50 crossed healthy weaning pigs [(Yorkshire × Landrace) × Duroc] with an average BW of 7.46 ± 1.60 kg (28 day of age) were used in a 6-week experiment. The experiment was divided into 3 phases (Phase 1, 1 - 2 weeks; Phase 2, 2 - 4 weeks; Phase 3, 4 - 6 weeks). Dietary treatments were as follows: 1) CON: basal diet and 2) CON + 0.50% YC. During phase 1, the average daily gain (ADG) and average daily feed intake (ADFI) were significantly increased (p < 0.05) in the weaning pigs fed YC supplementation diets compared with the weaning pigs fed the CON diet. During phase 3 as well as overall, the gain/feed ratio (G/F) was significantly increased (p < 0.05) in the YC supplementation group compared with the pigs fed the CON diet. In conclusion, the ation of YCs in the diet positively affected the growth performance of weaning pigs during the first two weeks after weaning.