Korean Journal of Agricultural Science

Article

, Vol. 45, Issue. 4

Analysis of intraspecific genetic diversity in Acidovorax citrulli causing bacterial fruit blotch on cucurbits in Korea

Jeong Young Song¹ May Moe Oo¹ Su Yeon Park¹ Mun Won Seo¹ Seong-Chan Lee² Nak Beom Jeon³ Myeong Hyeon Nam³ Youn Su Lee⁴ Hong Gi Kim^1,* Sang-Keun Oh^1,*

Department of Applied Biology, Chungnam National University, Daejeon 34134, Korea ¹
Protected Horticulture Research Institute, National Institute of Horticultural and Herbal Science, Haman 52054, Korea ²
Fruit and Vegetable Research Center, Chungnam ARES, Nonsan 32914, Korea ³
Division of Bio-resource Sciences, Kangwon National University, Chuncheon 24341, Korea ⁴

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Bacterial fruit blotch (BFB) caused by Acidovorax citrulli is a devastating disease found in many cucurbits cultivation fields. The genetic diversity for 29 strains of A. citrulli collected from various cucurbits in South Korea was determined by DNA fingerprinting with a pathogenicity test, multi locus analysis, Rep-PCR (repetitive sequence polymerase chain reaction), and URP (universal rice primers) PCR bands. Two distinct groups (Korean Clonal Complex, KCC1 and KCC2) in the population were identified based on group specific genetic variation in the multi locus phylogeny using six conserved loci and showed a very high similarity with DNA sequences for representative foreign groups [the group I (CC1-1 type) and the group II (CC2-5 type)] widely distributed worldwide, respectively. Additionally, in the case of phaC, a new genotype was found within each Korean group. The KCC1 was more heterogeneous compared to the KCC2. The KCC1 recovered mainly from melons and watermelons (ratio of 6 : 3) and 15 of the 20 KCC2 strains recovered from watermelons were dominant in the pathogen population. Accordingly, this study found that two distinct groups of differentiated A. citrulli exist in South Korea, genetically very similar to representative foreign groups, with a new genotype in each group resulting in their genetic diversity.

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