The purpose of this research was to produce symmetric (Saturated–Unsaturated–Saturated, SUS) triacylglycerol (TAG) using palm stearin fraction oil (PSFO) and high oleic sunflower oil (HOSO) as substrates to replace cocoa butter. PSFO was blended with HOSO (1 : 2 weight ratio), and lipozyme® TLIM (20 weight % of the substrate) was added. Interesterification was carried out in a shaking water bath at 55℃ at 220 rpm for 6 hours. The response surface methodology (RSM) through the central composite face design was employed to observe the optimized SUS-TAG. The independent factors were the reaction temperature (X : 65, 75 and 85℃), reaction time (X : 1, 3 and 5 hours) and ratio of TLIM (X : 10, 15 and 20 weight%). The dependent variables were Y = Saturated–Unsaturated–Unsaturated (SUU, area %), Y = SUS (area %), Y = Saturated–Saturated–Unsaturated (SSU, area %), Y = Unsaturated–Unsaturated–Unsaturated (UUU, area %), and Y = sn-2 unsaturated fatty acid (area %). The optimal conditions from the central composite face design minimized acyl migration while maximizing the presence of unsaturated fatty acid at the sn-2 position (73.43 area %). The optimal conditions were X= 65℃, X= 1 hour, and X= 20 weight%. As a result of the response surface analysis, the lack of fits was found as Y = 0.622, Y = 0.438, Y = 0.264, Y = 0.526, and Y = 0.215, and their R were 0.897, 0.944, 0.826, 0.857, and 0.867, respectively.