Safety assessment of the AtCYP78A7 protein expressed in genetically modified rice tolerant to abiotic stress
Kyong-Hee Nam1 Do Young Kim1 Hee Jae Shin1 In-Soon Pack1 Jung-Ho Park1 Won Kee Yoon2
Kim3 Chang-Gi Kim1,*
Bio-Evaluation Center, KRIBB, Cheongju 28116, Korea1 Laboratory Animal Resource Center, KRIBB, Cheongju 28116, Korea2 Life Sciences Research Institute, Biomedic Co., Ltd., Bucheon 14548, Korea3
Overexpression of AtCYP78A7, a gene encoding a cytochrome P450 protein, has been reported to improve tolerance to drought stress in genetically modified (GM) rice ( L.). The aim of this study was to evaluate the potential allergenicity and acute oral toxicity of the AtCYP78A7 protein expressed in GM rice. Bioinformatics analysis of the amino acid sequence of AtCYP78A7 did not identify any similarities with any known allergens or toxins. It showed that no known allergen had more than a 35% amino acid sequence homology with the AtCYP78A7 protein over an 80 amino acid window or more than 8 consecutive identical amino acids. The gene encoding the AtCYP78A7 protein was cloned in the pGEX-4T-1 vector and expressed in . Then, the AtCYP78A7 protein was purified and analyzed for acute oral toxicity. The AtCYP78A7 protein was fed at a dose of 2,000 mg/kg body weight in mice, and the changes in mortalities, clinical findings, and body weight were monitored for 14 days after the dosing. Necropsy was carried out on day 14. The protein did not cause any adverse effects when it was orally administered to mice at 2000 mg/kg body weight. These results indicate that the AtCYP78A7 protein expressed in GM rice would not be a potential allergen or toxin.
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Journal Title :
Korean Journal of Agricultural Science